Ecological Archives E090-246-A7

Thomas Larsen, D. Lee Taylor, Mary Beth Leigh, Diane M. O’Brien. 2009. Stable isotope fingerprinting: a novel method for identifying plant, fungal, or bacterial origins of amino acids. Ecology 90:3526–3535.

Appendix G. Linear discriminant analysis using 13C variations among six essential amino acids of the present study and those of Scott et al. (2006).

 
   FIG. G1. Linear discriminant analysis using 13C variations among six essential amino acids (Thr, Val, Leu, Ile, Phe, Lys) of fungi (N = 13), bacteria (N = 10), and plants (N = 10) from the present study and bacteria (N = 15) from Scott et al. (2006). Letters signify: s1, Escherichia coli (aerobic - pyruvate); s2, Escherichia coli (anaerobic - pyruvate); s3, Escherichia coli (acetate); s4, Shewanella oneidensis (aerobe - fumarate); s5, Shewanella oneidensis (aerobe - nitrate); s6, Shewanella oneidensis (anaerobe - fumarate); s7, Shewanella oneidensis (anaerobe - nitrate); s8, Desulfovibrio; s9, Methylophilus; s10, Methylobacterium (methylamine); s11, Methylobacterium (methanol); s12, Aquifex; s13, Clostridium acetobutylicum; s14, Thermotoga; s15, Desulfotomaculum. All bacteria from Scott et al. (2006) were classified as bacteria except for Desulfovibrio (s8), which was misclassified as a plant.

 

LITERATURE CITED

Scott, J. H., D. M. O'Brien, D. Emerson, H. Sun, G. D. McDonald, A. Salgado, and M. L. Fogel. 2006. An examination of the carbon isotope effects associated with amino acid biosynthesis. Astrobiology 6:867–880.


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