Ecological Archives E086-100-A1

**Spencer R. Hall, Val H. Smith, David A. Lytle, and Mathew A. Leibold. 2005. Constraints on primary producer N:P stoichiometry along N:P supply ratio gradients. Ecology 86:1894–1904.**

Appendix A. Details of the literature data set.

Table A1. Description of sources of literature data presented in Fig. 3.

Data set	No.^†	Reference^‡	Method^§	Supply^\|\|	Species^¶
Lakes	1	Stockner and Shortreed 1985	Obs.	Indirect	Lake assemblage
	2	Shortreed and Stockner 1986	Obs.	Indirect	Lake assemblage
	3	Healey and Hendzel 1980	Obs.	Indirect	Lake assemblage
	4	Sterner et al. 1995	Obs.	Indirect	Lake assemblage
	5	Suttle and Harrison 1985	Obs.	Indirect	Lake assemblage
Mesocosms	6	Levine and Schindler 1992	Expt. (O)	Direct	Lake assemblage
	7	Levine and Schindler 1997	Expt. (O)	Direct	Lake assemblage
	8	V. H. Smith et al. unpublished data	Expt. (O)	Direct	Pond assemblage
Algal	9	Saksaug et al. 1983	Cult.	Direct	Emiliania huxleyi, Amphidinium carterae
Cultures	10	Elrifi and Turpin 1985	Cult.	Direct	Selenastrum minutum
	11	Rhee and Lederman 1991	Cult.	Direct	Anabaena flos-aquae
	12	Rhee 1978	Cult.	Direct	Scenedesmus sp.
	13	Liu et al. 2000	Cult.	Direct	Aureoumbra lagunensis
	14	Reigmann et al. 2000	Cult.	Direct	Emiliana huxleyi
Periphyton	15	Hillebrand and Kahlert 2001	Expt. (O)	Indirect	Lake assemblage
	16	Stelzer and Lamberti 2002	Expt. (O)	Direct	Stream assemblage
	17	Stelzer and Lamberti 2001	Expt. (O)	Direct	Stream assemblage
Terrestrial and Wetland	18	Shaver and Melillo 1984	Expt. (L)	Direct	Carex lacustris, Calamagrostis canadensis, Typha latifolia
Plants	19	Güsewell in press	Expt. (O)	Direct	Carex flava, C. panicea, Molinia caerulea, Phalaris arundinacea
	20	Ryser and Lambers 1995	Expt. (O)	Direct	Brachypodium pinnatum, Dactylis glomerata
Marine	21	Doering et al. 1995	Obs.	Indirect	Marine assemblage
	22	Plum Island LTER (public comm.)	Obs.	Indirect	Marine assemblage
	23	US EPA EMAP (public comm.), MD and DE data sets	Obs.	Indirect	Marine assemblage
	24	US EPA EMAP (public comm.), MAIA data set	Obs.	Indirect	Marine assemblage

^† Data set number corresponds to that on Fig. 3.

^‡ References not included in the manuscript are listed below.

^§ Method by which data were collected: "Obs." = observations from natural systems; "Expt. (O)" = outdoors experiment; "Expt (L)" = laboratory experiment; "Cult." = continuous chemostat culture.

^|| Nature of nutrient supply. "Indirect" = approximating using ratio of total nitrogen:total phosphorus; "Direct" = directly controlled.

^¶ Identity of species if from a single-species culture or experiment; assemblage indicated that multiple species aggregated.

LITERATURE CITED

Doering, P. H., C. A. Oviatt, B. L. Nowicki, E. G. Klos, and L.W. Reed. 1995. Phosphorus and nitrogen limitation of primary production in a simulated estuarine gradient. Marine Ecology-Progress Series 124:271–287.

Elrifi, I. R., and D. H. Turpin. 1985. Steady-state luxury consumption and the concept of optimum nutrient ratios: a study with phosphate and nitrate limited Selenastrum minutum (Chlorophyta). Journal of Phycology 21:592–602.

Güsewell, S. In press. Responses of wetland graminoids to the relative supply of nitrogen and phosphorus. Plant Ecology.

Healey, F. P., and L. L. Hendzel. 1980. Physiological indicators of nutrient deficiency in lake phytoplankton. Canadian Journal of Fisheries and Aquatic Sciences 37:442–453.

Hillebrand, H., and M. Kahlert. 2001. Effect of grazing and nutrient supply on periphyton biomass and nutrient stoichiometry in habitats of different productivity. Limnology and Oceanography 46:1881–1898.

Liu, H., E. A. Laws, T. A. Villaral, and E. J. Buskey. 2001. Nutrient-limited growth of Aureoumbra lagunensis (Pelagophyceae), with implications for its capability to outgrow other phytoplankton species in phosphate-limited environments. Journal of Phycology 37:500–508.

PlumIsland LTER. Public communication. Accessed 12 June 2004. ecosystems.mbl.edu/pie

Reigman, R., W. Stolte, A. A. M. Noordeloos, and D. Slezak. 2000. Nutrient uptake and alkaline phosphatase (EC 3:1:3:1) activity of Emiliania huxleyi (Prymnesiophyceae) during growth under N and P limitation in continuous cultures. Journal of Phycology 36:87–96.

Rhee, G. -Y., and T. C. Lederman. 1983. Effects of nitrogen sources on P-limited growth of Anabaena flos-aquae. Journal of Phycology 19:179–185

Ryser, P., and H. Lambers. 1995. Root and leaf attributes account for the performance of fast and slow-growing grasses at different nutrient supply. Plant Soil 1670:251–265

Sakshaug, K,. Andersen, S. Myklestad, and Y. Olsen. 1983. Nutrient status of phytoplankton communities in Norwegian waters (marine, brackish, and fresh) as revealed by their chemical composition. Journal of Plankton Research 5:175–196.

Shaver, G. R., and J. M. Melillo. 1984. Nutrient budgets of marsh plants: efficiency concepts and relation to availability. Ecology 65:1491–1510.

Shortreed, K. S., and J. G. Stockner. 1986. Trophic status of 19 subarctic lakes in the Yukon Territory. Canadian Journal of Fisheries and Aquatic Sciences 43:797–805.

Stelzer, R. S., and G. A. Lamberti. 2001. Effects of N:P ratio and total nutrient concentration on stream periphyton community structure, biomass, and elemental composition. Limnology and Oceanography 46:356–367

Stelzer, R. S., and G. A. Lamberti. 2002. Ecological stoichiometry in running waters: periphyton chemical composition and snail growth. Ecology 83:1039–1051.

Sterner, R.W., T. H. Chranowski, J. J. Elser, and N. B. George. 1995. Sources of nitrogen and phosphorus supporting the growth of bacterio- and phytoplankton in an oligotrophic Canadian shield lake. Limnology and Oceanography 40:242–249.

Stockner, J. G., and K. S. Shortreed. 1985. Whole-lake fertilization experiments in coastal British Columbia lakes: empirical relationships between nutrient inputs and phytoplankton biomass and production. Canadian Journal of Fisheries and Aquatic Sciences 42:649–658.

Suttle, C. A., and P .J. Harrison. 1988. Ammonium and phosphate uptake rates, N:P supply ratios, and evidence for N and P limitation in some oligotrophic lakes. Limnology and Oceanography 33:186–202.

US EPA EMAP. Public communication. Accessed 12 June 2004. http://www.epa.gov/emap

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