Ecological Archives E088-011-A5

**Cynthia G. Hays. 2007. Adaptive phenotypic differentiation across the intertidal gradient in the alga Silvetia compressa. Ecology 88:149–157.**

Appendix E. ANOVA designs.

Replicated block ANOVAs for embryos outplanted at each of the three sites; the dependant variable is mean survival of each lineage replicated across outplant plates.

SCOTT’S CREEK

Source	Sum of squares	df	df_denominator	F	P
Parents’ height	0.315	1	2	1.92	0.30
Outplant height	0.014	1	2	0.031	0.88
Trial	0.219	2	204	1.26	0.29
Parents’ height × trial	0.328	2	204	1.88	0.16
Outplant height × trial	0.928	2	204	5.31	0.006*
Parents’ height × outplant height	1.007	1	2	183.21	0.005*
Parents’ ht × outplant tide ht × trial	0.001	2	204	0.063	0.94
Residual	17.816	204

GREYHOUND

Source	Sum of squares	df	df_denominator	F	P
Parents’ height	2.93	1	2	3025.4	<0.001*
Outplant height	0.194	1	2	93.24	0.011*
Trial	0.027	2	168	0.29	0.75
Parents’ height × trial	0.002	2	168	0.021	0.98
Outplant height × trial	0.004	2	168	0.045	0.96
Parents’ height × outplant height	1.068	1	2	80.93	0.012*
Parents’ ht × outplant tide ht × trial	0.026	2	168	0.284	0.75
Residual	7.794	168

PIGEON POINT

Source	Sum of squares	df	df_denominator	F	P
Parents’ height	0.037	1	2	6.83	0.12
Outplant height	3.17	1	2	3.858	0.19
Trial	0.152	2	168	0.138	0.14
Parents’ height × trial	0.011	2	168	1.879	0.87
Outplant height × trial	1.64	2	168	21.11	<0.001*
Parents’ height × outplant height	0.005	1	2	2.17	0.28
Parents’ ht × outplant tide ht × trial	0.004	2	168	0.054	0.95
Residual	6.54	168

Split-plot ANOVA for embryos from the three sites exposed to air in the lab; the dependant variable is the arcsin square-root transformed percent survival of embryos from each lineage, exposed to air for 3 h or 6 h.

SCOTT’S CREEK

Source	Sum of squares	df	df_denominator	F	P
Emersion time	0.717	1	28	20.40	<0.001*
Slide(emersion time)	0.985	28	28	2.30	0.016*
Parents’ height	0.281	1	28	18.32	<0.001*
Parents’ height × emersion time	0.005	1	28	0.316	0.579
residual	0.429	28

GREYHOUND

Source	Sum of squares	df	df_denominator	F	P
Emersion time	5.36	1	28	42.59	<0.001*
Slide(emersion time)	3.52	28	28	4.57	<0.001*
Parents’ height	0.218	1	28	7.913	0.009*
Parents’ height × emersion time	0.002	1	28	0.069	0.795
residual	0.772	28

PIGEON POINT

Source	Sum of squares	df	df_denominator	F	P
Emersion time	7.71	1	28	154.69	<0.001*
Slide(emersion time)	1.40	28	28	2.12	0.026*
Parents’ height	0.006	1	28	0.264	0.611
Parents’ height × emersion time	0.008	1	28	0.350	0.559
residual	0.658	28

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