Ecological Archives E090-221-A1

**Oscar Puebla, Eldredge Bermingham, and Frédéric Guichard. 2009. Estimating dispersal from genetic isolation by distance in a coral reef fish (Hypoplectrus puella). Ecology 90:3087–3098.**

Appendix A. Basic statistics per locus of 17 samples of the coral reef fish Hypoplectrus puella (Serranidae) genotyped at 10 microsatellite loci.

	Carrie B.	Utila	Guanaja	Becerros	Media L.	Bocas	Bocas	Bocas	Porvenir	Holand.	Puyadas	Ratones	Ingleses	Achutupu	Dupak	Goedup	Barb.
	n=50	n=55	n=55	n=55	n=54	n=50	n=35	n=35	n=47	n=54	n=53	n=55	n=55	n=50	n=46	n=55	n=50
	2004	2006	2006	2006	2006	2004	2005	2006	2005	2005	2005	2005	2005	2005	2005	2005	2005

Number of alleles
g2	21	23	18	20	24	21	20	16	20	18	23	20	21	20	22	21	21
gag010	23	21	23	24	21	19	19	20	20	17	18	23	22	22	19	24	18
h24	22	23	22	22	23	22	17	19	22	23	21	20	23	21	20	22	22
hyp001	9	7	10	9	8	9	7	10	7	9	8	7	8	9	9	8	8
hyp015	5	9	9	6	6	5	5	8	5	8	8	6	6	7	4	5	6
hyp018	6	6	5	5	6	5	6	5	5	4	6	4	4	5	5	5	7
e2	7	8	7	11	8	7	7	8	7	7	8	7	10	8	9	6	6
hyp008	21	24	25	21	27	22	18	18	24	28	28	32	29	28	26	26	15
hyp016	29	28	30	30	30	19	20	21	25	29	25	30	34	27	23	27	25
pam013	27	28	27	26	25	25	22	21	24	24	25	26	27	26	26	26	29
Mean	17	18	18	17	18	15	14	15	16	17	17	18	18	17	16	17	16

Allelic richness (El Mousadik and Petit 1997)
g2	18.2	19.5	15.9	17.8	19.1	18.4	19.5	16.0	17.7	15.6	18.8	17.7	18.1	17.4	20.0	17.2	18.8
gag010	20.2	17.6	19.9	20.4	18.6	18.3	18.7	19.2	17.8	15.9	17.4	20.2	19.1	19.7	17.3	20.5	16.5
h24	20.0	20.5	19.6	19.7	20.4	19.4	16.8	18.7	20.0	20.0	18.9	17.7	20.6	19.1	18.3	19.2	20.5
hyp001	8.5	6.9	8.8	8.6	7.9	8.3	7.0	9.7	7.0	8.3	7.6	7.0	7.6	8.9	8.7	7.8	7.7
hyp015	4.6	7.4	7.5	5.5	5.2	4.6	5.0	8.0	4.8	6.8	6.8	5.2	5.3	6.3	3.7	4.9	5.4
hyp018	5.3	5.4	4.6	4.5	5.7	4.4	5.9	4.8	4.4	4.0	5.1	3.9	4.0	4.8	4.7	4.6	6.2
e2	6.2	7.3	6.1	9.0	6.9	6.5	6.8	7.9	6.4	6.9	7.7	6.5	8.3	6.9	8.1	5.8	5.9
hyp008	18.3	20.7	19.8	18.2	21.9	19.1	17.8	17.8	22.0	25.3	24.2	27.0	24.7	24.5	23.3	22.3	14.1
hyp016	26.1	24.4	25.8	26.5	25.7	17.3	19.5	21.0	23.0	24.9	23.0	26.2	28.3	24.0	21.5	23.1	22.4
pam013	24.1	23.3	23.2	23.6	22.6	22.7	21.6	20.8	21.6	22.1	22.3	22.4	23.6	24.0	23.6	22.2	25.2
Mean	15	15	15	15	15	14	14	14	14	15	15	15	16	16	15	15	14

Gene diversity (Nei 1987)
g2	0.891	0.893	0.867	0.904	0.872	0.870	0.905	0.877	0.863	0.819	0.850	0.873	0.881	0.854	0.906	0.843	0.887
gag010	0.917	0.910	0.891	0.919	0.914	0.885	0.932	0.886	0.895	0.903	0.911	0.927	0.924	0.923	0.875	0.917	0.908
h24	0.939	0.940	0.940	0.943	0.940	0.923	0.910	0.932	0.920	0.934	0.938	0.923	0.928	0.939	0.922	0.916	0.941
hyp001	0.845	0.786	0.817	0.840	0.853	0.839	0.850	0.850	0.818	0.852	0.842	0.832	0.796	0.862	0.840	0.838	0.838
hyp015	0.593	0.584	0.623	0.554	0.516	0.532	0.523	0.688	0.528	0.578	0.518	0.515	0.474	0.648	0.377	0.510	0.615
hyp018	0.668	0.645	0.675	0.605	0.639	0.529	0.640	0.639	0.549	0.636	0.567	0.549	0.647	0.574	0.623	0.589	0.692
e2	0.589	0.609	0.588	0.609	0.668	0.667	0.596	0.536	0.521	0.649	0.596	0.570	0.496	0.539	0.529	0.510	0.604
hyp008	0.910	0.930	0.915	0.913	0.923	0.913	0.876	0.869	0.948	0.951	0.955	0.956	0.951	0.955	0.950	0.949	0.912
hyp016	0.956	0.946	0.960	0.959	0.956	0.911	0.897	0.924	0.944	0.954	0.954	0.951	0.955	0.948	0.936	0.932	0.951
pam013	0.949	0.943	0.946	0.946	0.953	0.951	0.932	0.942	0.944	0.950	0.949	0.946	0.951	0.945	0.944	0.944	0.955
Mean	0.826	0.819	0.822	0.819	0.823	0.802	0.806	0.814	0.793	0.823	0.808	0.804	0.800	0.819	0.790	0.795	0.830

Fis (Weir and Cockerham 1984)
g2	–0.055	–0.012	–0.040	–0.044	0.048	–0.058	0.022	–0.036	0.014	0.024	0.001	0.049	–0.025	–0.001	–0.006	–0.056	–0.060
gag010	–0.003	0.001	0.013	0.037	0.032	0.022	–0.012	0.054	0.035	–0.063	–0.043	0.005	–0.017	0.101	–0.035	0.014	0.037
h24	–0.001	–0.003	–0.004	–0.041	0.016	0.025	0.058	–0.073	–0.018	0.034	0.055	0.121	0.024	0.000	–0.084	–0.052	0.001
hyp001	0.100	0.010	–0.083	0.096	0.004	0.003	–0.008	0.015	–0.118	–0.056	–0.098	0.029	–0.043	0.005	0.053	–0.063	0.046
hyp015	0.157	–0.027	–0.121	–0.104	0.050	0.174	–0.147	–0.251	0.033	0.118	0.072	0.028	–0.076	–0.029	0.076	0.215	–0.040
hyp018	–0.161	0.154	0.032	–0.072	0.014	–0.024	0.127	–0.142	0.030	–0.048	0.051	–0.114	–0.021	0.057	0.038	–0.164	–0.011
e2	–0.053	–0.095	0.037	–0.075	0.057	0.100	–0.055	–0.153	–0.103	0.003	0.006	–0.022	–0.063	0.147	–0.109	0.072	–0.048
hyp008	–0.076	–0.036	–0.032	–0.015	0.077	0.036	–0.108	–0.049	0.129	–0.052	0.038	0.052	0.048	0.078	0.062	0.099	0.013
hyp016	–0.026	–0.057	–0.003	0.020	–0.046	–0.032	–0.051	0.016	0.035	–0.028	0.018	0.033	–0.027	–0.034	–0.017	0.051	–0.030
pam013	0.009	0.055	–0.037	0.100	–0.006	0.034	–0.012	–0.030	–0.014	–0.030	–0.009	0.020	0.006	–0.007	–0.060	0.000	–0.005
All	–0.012	–0.002	–0.024	–0.001	0.022	0.022	0.007	–0.014	0.006	0.028	–0.013	0.028	–0.011	0.007	–0.008	–0.016	–0.054

Proportion of randomisations that gave a larger Fis than the observed (before Bonferroni correction for multiple tests):
g2	0.935	0.685	0.845	0.915	0.200	0.937	0.429	0.810	0.475	0.404	0.571	0.197	0.784	0.583	0.641	0.924	0.955
gag010	0.616	0.564	0.471	0.240	0.288	0.426	0.706	0.227	0.306	0.968	0.893	0.530	0.749	0.023	0.818	0.440	0.269
h24	0.598	0.629	0.631	0.960	0.400	0.340	0.194	1.000	0.756	0.244	0.108	0.005	0.333	0.582	1.000	0.957	0.583
hyp001	0.073	0.499	0.939	0.077	0.534	0.542	0.621	0.490	0.984	0.879	0.980	0.378	0.801	0.527	0.255	0.904	0.279
hyp015	0.091	0.671	0.936	0.914	0.379	0.067	0.941	0.999	0.443	0.131	0.293	0.448	0.834	0.682	0.326	0.024	0.719
hyp018	0.979	0.059	0.416	0.818	0.498	0.655	0.163	0.926	0.453	0.745	0.372	0.923	0.647	0.340	0.414	0.983	0.608
e2	0.779	0.926	0.395	0.893	0.286	0.142	0.774	0.973	0.919	0.554	0.542	0.669	0.868	0.066	0.957	0.247	0.787
hyp008	0.992	0.914	0.860	0.713	0.047	0.261	0.993	0.877	0.002	1.000	0.183	0.082	0.111	0.024	0.076	0.006	0.452
hyp016	0.903	1.000	0.643	0.335	1.000	0.848	0.915	0.472	0.236	0.914	0.376	0.221	0.916	0.934	0.761	0.135	0.918
pam013	0.479	0.092	0.951	0.006	0.679	0.218	0.718	0.873	0.753	0.910	0.706	0.347	0.516	0.677	1.000	0.583	0.661
All	0.771	0.553	0.916	0.515	0.092	0.107	0.338	0.806	0.366	0.043	0.795	0.051	0.731	0.320	0.660	0.767	0.997

Proportion of randomisations that gave a smaller Fis than the observed (before Bonferroni correction for multiple tests):
g2	0.171	0.511	0.298	0.212	0.901	0.165	0.777	0.401	0.708	0.757	0.614	0.903	0.391	0.606	0.584	0.174	0.137
gag010	0.601	0.626	0.712	0.883	0.850	0.772	0.568	0.899	0.843	0.113	0.284	0.678	0.466	0.993	0.363	0.743	0.862
h24	0.638	0.606	0.602	0.174	0.787	0.824	0.922	0.072	0.464	0.887	0.957	0.998	0.827	0.653	0.023	0.141	0.657
hyp001	0.966	0.636	0.130	0.961	0.621	0.616	0.570	0.687	0.048	0.235	0.058	0.751	0.322	0.638	0.857	0.187	0.830
hyp015	0.952	0.456	0.121	0.167	0.742	0.968	0.152	0.005	0.696	0.931	0.818	0.689	0.296	0.452	0.813	0.990	0.409
hyp018	0.048	0.970	0.703	0.283	0.627	0.496	0.920	0.147	0.684	0.372	0.741	0.152	0.477	0.781	0.719	0.043	0.523
e2	0.354	0.154	0.739	0.209	0.819	0.924	0.397	0.105	0.186	0.605	0.629	0.485	0.284	0.972	0.134	0.861	0.360
hyp008	0.049	0.239	0.306	0.483	0.983	0.869	0.051	0.305	1.000	0.102	0.929	0.972	0.959	0.993	0.976	0.998	0.728
hyp016	0.337	0.051	0.633	0.856	0.085	0.329	0.261	0.762	0.902	0.307	0.828	0.912	0.308	0.256	0.499	0.945	0.295
pam013	0.751	0.966	0.208	0.998	0.595	0.910	0.570	0.406	0.506	0.315	0.560	0.827	0.717	0.604	0.071	0.652	0.618
All	0.231	0.448	0.084	0.485	0.909	0.893	0.663	0.194	0.634	0.957	0.206	0.949	0.270	0.681	0.344	0.242	0.004

LITERATURE CITED

El Mousadik, A., and R. J. Petit. 1996. Theor. Appl. Genet. 92:832–839.

Nei, M. 1987. Molecular Evolutionary Genetics. Columbia University Press, New York, New York, USA.

Weir, B. S., and C. C. Cockerham. 1984. Evolution 38:1358–1370.

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